Μελέτη των επιπέδων των γλυκοζαμινογλυκανών και αυξητικών παραγόντων (CTGF και VEGF) στον σακχαρώδη διαβήτη τύπου Ι: συσχέτισή τους με δείκτες φλεγμονής και την νεφρική λειτουργία

Abstract

Aim To evaluate the role of extracellular matrix-associated (Glycosaminoglycans - GAGs, tissue growth factor - CTGF), angiogenic (VEGF) and inflammatory factors (MCP-1, CD40, IFN-β) in the development of diabetic nephropathy in type 1 diabetes (T1DM). Materials and Methods Plasma and urine samples from 30 T1DM patients and 20 healthy controls were used to measure the levels of CTGF, VEGF, MCP-1, CD40 and IFN-β by ELISA. Plasma and urine GAGs were measured using a spectrophotometric method. Results Plasma levels of GAGs and CD40 and MCP-1 and also urine levels of GAGs and CTGF were significantly elevated in normoalbuminuric T1DM patients. A tendency to higher plasma VEGF levels was found in patients compared to controls. The urine/plasma GAGs ratio of T1DM patients was almost similar to that of healthy subjects, whereas the urine/plasma CTGF ratio was about three times greater in diabetic patients compared to healthy subjects. Conclusion Increased GAGs and CTGF excretion are evident in T1DM normalbuminuric juveniles, possibly reflecting early renal injury signs, before the initiation of albuminuria.