Ανάπτυξη και εφαρμογή βιοφωταυγών κυτταρικών βιοαισθητήρων για εκτίμηση τοξικότητας με την τεχνική εισαγωγής δείγματος σε ροή
Abstract
In accordance with the legislation of the European Union the quality of water and the degree of contamination is assessed by chemical methods. However, these methods in contrast with bioassays, do not account for synergistic and competitive actions which could affect bioavailability and consequently the toxicity of different classes of pollutants. Bioassays may be used for assessment of toxicity as preliminary semiquantitative measurements before carrying out more detailed chemical analyses. Moreover, a large number of routine analysis can be performed rapidly and reliably through the development of automated analytical systems. A special benefit concerns the development of portable analytical instrumentation. In this way samples are not transported in the laroratory; the laboratory moves to the sampling area. In the present PhD thesis, the development and implementation of bioluminescent whole cell biosensors, for assessment of toxicity, fludic system is described. Biosensors are base ...
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