Βιοχημική και μοριακή μελέτη του εξωεμβρυικού υγρού

Abstract

THE PRESENT STUDY HAS TAKEN PLACE IN THE OBSTETRICS AND GYNAECOLOGY DEPARTMENTOF THE UNIVERSITY HOSPITAL OF IOANNINA, GREECE FROM 1/1/1994 TO 10/1 /1997. THE PURPOSE OF THIS STUDY WAS TO STUDY EMBRYONIC PHYSIOLOGY AT THE BEGGININGOF THE GESTATION AND TO EVALUATE THE POSSIBITY OF COELOCENTESIS FOR PRENATAL DIAGNOSIS OF SINGLE GENE DEFECTS AS B-THALASSEMIA. COELOCENTESIS AND/OR AMINOCENTESIS WERE PERFORMED IN 47 PREGNANT WOMEN ON THE 7-11TH WEEK OF GESTATION. THE PATIENTS ATTENDED THE OBSTETRICS OFFICE OF THE DEPARTMENT FOR TERMINATION OF PREGNANCY FOR PHYCHOLOGICAL REASONS. COELECE-NTESIS WAS PERFORMED UNDER GENERAL ANESTHESIA UNDER CONTINUOUS TRANSVAGINAL ULTRASONOGRAPHIC GUIDANCE. ASPIRATION OF COELOMIC FLUID AND ASPIRATION OF AMNIOTIC FLUID USING A NEW NEEDLE WERE PERFORMED SEQUENTIALLY. NETROMYCIN (NETI-LMICIN SULPHATE 300MG) WAS GIVEN INTRAVENOUSLY IN 23 WOMEN AS CHEMOPROPHYLAXIS 1-2 HOURS PRIOR TO THETERMINATION OF PREGNANCY. AFTERWARDS ASPIRATION OF COELOMIC AND/OR AMNIOTIC FLUID WERE PERFORMED. IN 19 OF THE 23 WOMEN FROM WHOM COELOMIC AND AMNIOTIC FLUIDS WERE ASPIRATED THE CONCENTRATIONS OF NETILMYCIN WERE EVALUATED IN THE MATERNAL SERUM, IN THE SAMPLES OF COELOMIC AND AMNIOTIC FLUIDS. THE CONCENTRATIO OF NETILMICIN IN THE MATERNAL SERUM WAS MUCH HIGHER THAN IN THE COELOMICAND AMNIOTIC FLUID AND ITS CONCENTRATIONS WAS HIGHER IN THE COELOMIC THAN INTHE AMNIOTIC FLUID. IN 20 WOMEN, THE TOTAL PROTEIN AMOUNT WAS MEASURED IN THE SAMPLES OBTAINED BY COELOCENTESIS AND AMNIOCENTESIS. (ABSTRACT TRUNCATED)

Η ΠΑΡΟΥΣΑ ΜΕΛΕΤΗ ΠΡΑΓΜΑΤΟΠΟΙΗΘΗΚΕ ΣΤΗΝ ΜΑΙΕΥΤΙΚΗ ΚΑΙ ΓΥΝΑΙΚΟΛΟΓΙΚΗ ΚΛΙΝΙΚΗ ΤΟΥΠΑΝΕΠΙΣΤΗΜΙΟΥ ΙΩΑΝΝΙΝΩΝ, ΚΑΤΑ ΤΟ ΧΡΟΝΙΚΟ ΔΙΑΣΤΗΜΑ 1/1/1994 ΕΩΣ 1/10 /1997. Ο ΣΚΟΠΟΣ ΤΗΣ ΕΡΓΑΣΙΑΣ ΗΤΑΝ ΝΑ ΜΕΛΕΤΗΘΕΙ Η ΕΜΒΡΥΙΚΗ ΦΥΣΙΟΛΟΓΙΑ ΣΤΗΝ ΑΡΧΗ ΤΗΣ ΚΥΗΣΗΣ ΚΑΙ ΝΑ ΕΞΕΤΑΣΘΕΙ Η ΔΥΝΑΤΟΤΗΤΑ ΤΗΣ ΧΡΗΣΗΣ ΤΗΣ ΠΑΡΑΚΕΝΤΗΣΗΣ ΤΗΣ